The CRAC Channel: Methods and Protocols (Methods in Molecular Biology, 1843)

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Management number 233410461 Release Date 2026/06/27 List Price US$23.69 Model Number 233410461
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This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, The CRAC Channel: Methods and Protocols is a valuable resource for any researcher interested in learningmore about the CRAC channel, as its activation mechanism and roles in cellular functions have yet to be completely explored. Read more

ASIN 149398702X
ISBN10 9781493987023
ISBN13 978-1493987023
Edition 1st ed. 2018
Language English
Publisher Humana
Dimensions 7.25 x 0.75 x 10.5 inches
Item Weight 1.32 pounds
Print length 215 pages
Publication date September 10, 2018

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